First make sure that you don't have any error when you click on the analyze button.
If no error, you may have hit a issue seen with recent version of STM32CubeMX where the middleware directory is deleted upon project regeneration.
To workaround the issue, uncheck the checkbox "Delete previously generated files when not regenerated" under Project Manager -> Code Generator -> Generated Files
In X-CUBE-AI 8.1 we make sure to restore the deleted files if they are needed post code generation by STM32CubeMX
Regards
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