Actually, this issue here refer to the setting of "TrustZone". If I negative this option when starting the project, the HAL_Delay worked. Why does this setting impact the Systick? 

Additional Info:HAL_Delay() stuck at while ((HAL_GetTick() - tickstart) < wait) { }and uwTick always equal to 0, __weak uint32_t HAL_GetTick(void) { return uwTick; } 

Sarrs.S ,thank you.I checked that the HAL_InitTick return OK. The tickStart can't get the right value to, It always equal to 0 or 0xffffffff..And in order to check the Internal Clock, I try to run a 50ms TIM2, but it stilled failed.