Did you upgrade CubeMX to 6.0.0 as well? I experienced the same issue after any change to the .ioc file. If a program is generated in TouchGFX and then run right to the target F469i-Disco board, it functions. I can also open it in Cube IDE 1.4.1 a...

So after some more fiddling around, there is a reference to CMSIS_RTOS (not the _V2 version) on, for me at least, line 144 of my .cproject file, and one on line 38 of the subdir.mk file. You can do a file search for CMSIS_RTOS and it will find all o...

I just upgraded to Cube MX 6.0.0, TouchGFX 4.14.0 and CubeIDE 1.4.1 and noticed this as well. I believe the release notes made mention to FreeRTOS not being required with some changes to CMSIS_V1 and CMSIS_V2, not quite sure how that all works, but ...