Multi-channel on STM32F429DISCO not working

Are you sure the pin is free to use? Very few pins on that board.

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Show the code, especially all related to initialization of pins and ADC

On CubeMX the pin is free.

static void MX_ADC1_Init(void)
{
 
 /* USER CODE BEGIN ADC1_Init 0 */
 
 /* USER CODE END ADC1_Init 0 */
 
 ADC_ChannelConfTypeDef sConfig = {0};
 
 /* USER CODE BEGIN ADC1_Init 1 */
 
 /* USER CODE END ADC1_Init 1 */
 
 /** Configure the global features of the ADC (Clock, Resolution, Data Alignment and number of conversion)
 */
 hadc1.Instance = ADC1;
 hadc1.Init.ClockPrescaler = ADC_CLOCK_SYNC_PCLK_DIV4;
 hadc1.Init.Resolution = ADC_RESOLUTION_12B;
 hadc1.Init.ScanConvMode = ENABLE;
 hadc1.Init.ContinuousConvMode = DISABLE;
 hadc1.Init.DiscontinuousConvMode = DISABLE;
 hadc1.Init.ExternalTrigConvEdge = ADC_EXTERNALTRIGCONVEDGE_NONE;
 hadc1.Init.ExternalTrigConv = ADC_SOFTWARE_START;
 hadc1.Init.DataAlign = ADC_DATAALIGN_RIGHT;
 hadc1.Init.NbrOfConversion = 2;
 hadc1.Init.DMAContinuousRequests = DISABLE;
 hadc1.Init.EOCSelection = ADC_EOC_SINGLE_CONV;
 if (HAL_ADC_Init(&hadc1) != HAL_OK)
 {
 Error_Handler();
 }
 
 /** Configure for the selected ADC regular channel its corresponding rank in the sequencer and its sample time.
 */
 sConfig.Channel = ADC_CHANNEL_0;
 sConfig.Rank = 1;
 sConfig.SamplingTime = ADC_SAMPLETIME_15CYCLES;
 if (HAL_ADC_ConfigChannel(&hadc1, &sConfig) != HAL_OK)
 {
 Error_Handler();
 }
 
 /** Configure for the selected ADC regular channel its corresponding rank in the sequencer and its sample time.
 */
 sConfig.Channel = ADC_CHANNEL_1;
 sConfig.Rank = 2;
 if (HAL_ADC_ConfigChannel(&hadc1, &sConfig) != HAL_OK)
 {
 Error_Handler();
 }
}

And this is when I start the ADC : (My project is combine with a Touchgfx project)

void Model::tick()
{
	tickcounter++;
 
	if(tickcounter % 63 == 0)
	{
		HAL_ADC_Start(&hadc1);
		HAL_ADC_Start_DMA(&hadc1, ADC_Val, 2);
 
		modelListener->updateTempGraph();
	}
}

Regards