STM32L4 Can JTAG pins be re-used after initial firmware downlod?

[TBoya.1]

I am trying to use the JTAG pins for other purposes if possible.

Is there a way to separate both programming and functional modes?

[Tesla DeLorean]

Yes, you can reconfigure the pins, disable the interface, number of options there.

Least invasive might be use for buttons. Could also use for LEDs.

Would recommend giving at least a brief window after reset and initialization for the debugger to be able to wrestle control, otherwise "Connect Under Reset" and "BOOT0 High" methods might be needed to gain control.